effects of l-carnitine on cardiac apoptosis in ischemic- reperfused isolated rat heart
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abstract
carnitine is a vital biologic substance for transporting fatty acids into myocytes. it also facilitates fatty acids β-oxidation for energy production. in this study, effects of l-carnitine (l-car) on apoptosis in the ischemic isolated rat heart were investigated. male sprague-dawley rats were divided into four groups and anesthetized by sodium pentobarbital. the heart was removed and mounted on a langendorff apparatus then perfused by a modified krebs-henseleit (k/h) solution under a constant pressure at 37 °c. in the control group, the hearts were perfused only by normal k/h solution at stabilization, 30 min. regional ischemia and 120 min. reperfusion, while in each of the test groups, the hearts were perfused during ischemia-reperfusion with 0.5, 2.5 and 5 mm of l-car-enriched k/h solution, respectively. at the end of reperfusion, immunohistochemical detection of apoptotic cells was performed by using an in situ apoptosis detection kit. the number of tunel-positive cardiomyocytes was counted in five random high-power fields in each sample. in the control group, the number of apoptotic cells were 48±3 while addition of l-car (0.5, 2.5 and 5 mm) to the solution reduced the number of apoptotic cells to 6±1, 4±1 and 3±1, respectively (p
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Journal title:
iranian journal of pharmaceutical sciencesجلد ۳، شماره ۱، صفحات ۱۹-۲۴
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