effects of l-carnitine on cardiac apoptosis in ischemic- reperfused isolated rat heart

Authors

moslem najafi

department of pharmacology, faculty of pharmacy, research center for pharmaceutical nanotechnology, tabriz university of medical sciences, tabriz, iran alireza garjani

department of pharmacology, faculty of pharmacy yousef dustar

faculty of veterinary medicine, islamic azad university of tabriz, tabriz, iran

abstract

carnitine is a vital biologic substance for transporting fatty acids into myocytes. it also facilitates fatty acids β-oxidation for energy production. in this study, effects of l-carnitine (l-car) on apoptosis in the ischemic isolated rat heart were investigated. male sprague-dawley rats were divided into four groups and anesthetized by sodium pentobarbital. the heart was removed and mounted on a langendorff apparatus then perfused by a modified krebs-henseleit (k/h) solution under a constant pressure at 37 °c. in the control group, the hearts were perfused only by normal k/h solution at stabilization, 30 min. regional ischemia and 120 min. reperfusion, while in each of the test groups, the hearts were perfused during ischemia-reperfusion with 0.5, 2.5 and 5 mm of l-car-enriched k/h solution, respectively. at the end of reperfusion, immunohistochemical detection of apoptotic cells was performed by using an in situ apoptosis detection kit. the number of tunel-positive cardiomyocytes was counted in five random high-power fields in each sample. in the control group, the number of apoptotic cells were 48±3 while addition of l-car (0.5, 2.5 and 5 mm) to the solution reduced the number of apoptotic cells to 6±1, 4±1 and 3±1, respectively (p

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Journal title:
iranian journal of pharmaceutical sciences

جلد ۳، شماره ۱، صفحات ۱۹-۲۴

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